Bio Rad Western Blot Box

Learn more about western blotting techniques.

Bio rad western blot box.

Western blotting products include the v3 western workflow protein transfer systems membranes filter paper blotting buffers and detection kits. Western blotting also known as immunoblotting or protein blotting is a core technique in cell and molecular biology. Put protein to be loaded into a 1 5 ml tube and add sample buffer to reach 1x concentration. Bio rad s fluorescent western blotting workflow is a seamless integration of products designed to work together to offer guaranteed results.

A special section the western blot doctor is a self help guide developed by bio rad researchers that enables you to identify and troubleshoot western blotting problems. Find step by step protocols and helpful tips on equipment membranes transfer conditions and detection methods. See how the trans blot turbo transfer system fits into the v3 western workflow to streamline your western blotting process. Protein separation and analysis.

Boil tubes at 100 c for 5 minutes. Use bio rad s precision plus protein westernc standards and clarity western ecl substrate for chemiluminescence detection on both film and digital imagers. Store at 20 c until ready for blotting. Bio rad s western blotting products include the v3 western workflow system wet semi dry and rapid protein transfer systems blotting membranes filter paper and buffers protein standards reagents and detection kits and lab validated western blotting antibodies.

In most basic terms it is used to detect the presence of a specific protein in a complex mixture extracted from cells or tissue. Western blotting this protocol uses the bio rad blotting system. Comprehensive solutions and suggestions are provided to help solve your particular western blotting challenges.